For over 2,000 years, Astragalus membranaceus (Huangqi) has been revered in Traditional Chinese Medicine as the "Supreme Qi-Tonifying Herb." Modern science now validates its legendary status through cycloastragenol (CAG)—a bioactive tetracyclic triterpenoid that exemplifies nature’s power to combat aging, immune dysfunction, and chronic disease. As a premier manufacturer of plant extracts, we unlock this potent compound using cutting-edge purification technologies to deliver >98% purity for global health innovations.

Botanical Origin and Chemical Profile

Cycloastragenol is the aglycone of astragaloside IV, isolated from the roots of Astragalus membranaceus (Fisch.) Bge. var. mongholicus. This small molecule (C??H??O?, MW: 490.72) features a unique 9,19-cyclolanostane structure with four hydroxyl groups critical to its bioactivity. Our extraction process preserves its integrity through:

• Ethanol/water gradient extraction followed by chromatographic purification

• Strict QC protocols: HPLC-DAD/ELSD validation, NMR and mass spectrometry confirmation7

• Stability optimization: -20°C storage in amber vials to prevent degradation

Table 1: Key Specifications of Our Cycloastragenol

Dual Mechanisms of Action: From Telomeres to Tumor Immunity

1. Telomerase Activation & Anti-Aging Effects

Cycloastragenol is nature’s first documented telomerase activator. By binding the catalytic site of telomerase reverse transcriptase (TERT), it:

• Extends telomeres in CD4+/CD8+ T-cells, delaying replicative senescence

• Upregulates tert and bcl2 mRNA in neuronal cells, protecting against oxidative stress

• Enhances antiviral immunity by 300% in human T-lymphocytes (in vitro)

This mechanism underpins its potential in age-related disorders—from skin rejuvenation to neuroprotection.

2. Immuno-Oncology: Unblocking MHC-I Antigen Presentation

Groundbreaking 2022 research revealed cycloastragenol’s cancer-fighting secret: it directly targets cathepsin B (CTSB) at residues A77/G198. This interaction:

• Blocks MHC-I degradation in lysosomes, increasing tumor antigen presentation

• Elevates immunogenic genes (*H2-k1*, B2m, CD74) by 3–5-fold per scRNA-seq data

• Synergizes with anti-PD-1 therapy, shrinking colon tumors by 60% vs. monotherapy in murine models

• "CAG reshapes the tumor microenvironment—it’s a ‘checkpoint inhibitor enhancer’ for cold tumors."

— Journal for Immunotherapy of Cancer, 2022

Applications in Modern Therapeutics

Validated in peer-reviewed studies, cycloastragenol holds promise for:

• Age-delay supplements: Doubles telomerase activity at 1 μM (6-day exposure

• Oncology adjuvants: 10 mg/kg/day dosing with PD-1 antibodies boosts CD8+ T-cell infiltratio

• Neuroprotective agents: Induces CREB phosphorylation to alleviate depression-like behaviors

• Anti-inflammatory formulations: Suppresses NLRP3 inflammasomes in psoriasis models

Quality Commitment

We ensure pharmaceutical-grade cycloastragenol through:

• Bioactivity assays: Telomerase activation (TRAP assay), CTSB binding (MST validation)

• Traceability: Sustainable sourcing from GAP-certified Astragalus farms

• Scalability: 50–1000 kg batches with COA, stability data, and customs documentation

The Future Is Natural

Cycloastragenol bridges ancient wisdom and 21st-century science. As clinical trials explore its potential in longevity and combo oncology therapies, we provide the purest, most bioactive CAG to fuel breakthroughs. Partner with us to co-develop formulations that redefine healthspan